The DCM
extract was dissolved in absolute ethanol (200 mg/ml) and diluted
with H2O containing 2 mg/ml fatty acid–free BSA (Bovine serum
albumin; Sigma, St. Louis, MO) as a stock solution at 10 mg/ml.
In the treatment, the final concentration of ethanol was less than
1%. The MeOH extract was dissolved in the medium of each cell
line (10 mg/ml) containing 0.2% dimethyl sulphoxide (DMSO) (Sigma).
In every treatment, the final concentration of DMSO was less
than 0.1%.