2.2.5. Total phenolics content (TPC)
Total phenolics were determined by following the method of
Singleton and Rossi (1965). A 0.5 g of SI defatted sample was
homogenised with 10 ml of 70% acetone to a uniform consistency
and left at 4 C for 20 h before filtration. Five hundred ll of samples
were combined with 1,250 ll of a 7.5% sodium carbonate solution
and 250 ll of 1 N Folin–Ciocalteu reagent and allowed to react for
30 min at room temperature. Absorbance of the mixture was measured
at 755 nm. Gallic acid was used as a standard. TPC’s were expressed
as milligram of gallic acid equivalents (GAE)/100 g of seed.