The pectin extracted was analyzed for equivalent weight,
methoxy content and galacturonic acid (Ranganna, 1995). Galacturonic
acid content of pectin samples was estimated by the
modified uronic acid carbozole reaction as recommended by Bitter
and Muir (1962). Standard galacturonic acid Sigma AR grade was
used to prepare standard solutions containing 10–50 mg galacturonic
acid and a standard curve was drawn.