Growth and preparation for biosorption
The biosorbent used throughout this study, Ochrobactrum sp.
GDOS, was isolated from area on private property in north of Iran,
where there was a times gone by herbicide dealing. The isolate was
identified according to the biochemical and molecular (16S rRNA
gene sequence) methods. It was cultured in 5 ml nutrient broth for
12 h at 30 8C and 180 rpm. 200 ml of cultures were used to
inoculate 500 mL nutrient broth in 2000 mL Erlenmeyer flask and
incubated at 30 8C with constant shaking at 180 rpm for 16 h. The
cells was then harvested by centrifugation for 15 min at 7000 rpm
and rinsed three times with sterile distilled water and freeze dried.