PCR-RFLP
PCR-RFLP is a variation of RFLP in which the
restriction analysis is performed on PCR amplicons
obtained using primers for specific
sequences of interest. A DNA banding profile
is generated cutting the PCR amplicons with
restriction enzymes. To generate adequate discrimination
after cutting the amplicon, the
amplified gene needs to have variable sequences
flanked by highly conserved regions to be
targeted by universal primers. This method can
identify a genetic correlation among pathogenic
bacteria and is used in molecular epidemiology
study of different genera and species (ElAdawi
et al., 2013; Pereyre et al., 2013).