The focus of this research was the simultaneous saccharification and fermentation where S. dimorphus is able to accumulate large amount of starch compared to other biochemical composition. Lipid-extracted biomass is directly subjected to SSF without any energy-intensive pretreatment. In this regards, optimization on the reaction will be studied in order to determine the most suitable condition for single enzyme in saccharification to convert starch content to fermentable sugar while maintaining an optimum condition for the yeast fermentation process to occur.