Abstract:
Reticulocytes are young non-nucleated red cells which retain RNA within the cell. The reticulocyte count is useful to
indicate marrow erythropoietic activity and also to classify the causes of anemia disease. The conventional method for enumerating
percentage of reticulocytes is by staining with supravital stain by 0.5% New Methylene Blue and incubating at room temperature
(25๐
C) within 1 hour. With an attempt to reduce the testing time, we studied the percentage of reticulocyte by increasing the
temperature (37๐
C) by reducing the incubation time (5, 10 and 15 minutes) compared with the conventional method. The
results showed no significant difference of the three methods compared with conventional method with p=0.67, 0.06, 0.15
respectively. There was close correlation between 3 conditions and conventional method with r=0.994, 0.996, 0.996