Optimally, the fragment of interest should be no greater than 2-3 kilobases (kb) longer than the core region, which is a limitation imposed by the size of a region that can be efficiently amplified using presently available methods of PCR.
Optimally, the fragment of interest should be no greater than 2-3 kilobases (kb) longer than the core region, which is alimitation imposed by the size of a region that can be efficiently amplified using presently available methods of PCR.