Spirulina is one of the most explored cyanobacteria. Since ancient time it is being used as source of protein. Spirulina pletensis was cultivated in different medium like; zarrouk’s medium, CFTRI medium, OFERR medium, zarrouk’s medium + PGR medium, agitation and without agitation medium, improved VITAL BIOTECH medium. Different temperature, light intensity and pH were monitored for 20 days on daily basis. pH was found in range from 9.1 to 10.1in different medium. Gradually increase in dry weight (dw) was noticed along with the age of culture, 0.40-1.25 dw/l was achieved in different medium respectively. Spirulina inoculated in improved VITAL BIOTECH medium was survived and growth was flourished, achieving dry weight of 0.82 dw/l on 20th day of cultivation. Different amount of NaHCO3 and NaNO3 shown significant impact on Spirulina growth. However results of present investigation could be consider for commercial cultivation of Spirulina using different physical and chemical environment for mass production of Spirulina pletensis.