2.6. Statistical analyses
In the laboratory safety study, the rectal temperatures after vaccination
were compared in a 2-sample t-test. Rectal temperatures
in the field safety study were analyzed using a repeated measures
ANOVA model with farm and interaction farm treatment group
as random effects, treatment group as fixed effect and mean
pre-vaccination temperature as covariate.
Lung lesion scores in challenge experiments and the qPCR data
of inguinal and mesenteric lymph nodes, lung and tonsil were analyzed
by the Wilcoxon Rank Sum test. PCV2 serology data at 2 wpv
(OOI study) were analyzed by ANOVA and the serology in the time
period 0–22 wpv (DOI study) was analyzed by ANOVA for repeated
measurements. The PCV2 serology in the field study was also analyzed
by repeated measures ANOVA.