2.2.3.4. Transmission electron microscope
Transmission electron microscopy (TEM) technique was used to visualize the morphology of the CPL-AgNPs. The make of Transmission electron microscope (TEM; Philips model CM 200) technique was to visualize the morphology of nanoparticles. The instrument was operated at an accelerating voltage of 200 kV with ultra-high-resolution of 0.2 nm and magnification of 2,000,000 X. TEM’s grid size is 3 mm diameter which was prepared placing a 5 μL of the silver nanoparticles solutions on carbon-coated copper grids and drying under mercury lamp and then analysed. The size of the silver nanoparticles can be determined by using the image magnifying software and this software will be able to magnify the particles with size less than 10 nm and give clear morphological data.