Two globally known fruits, Citrus limon (lemon) and Punicagranatum L. (pomegranate), were collected from the farms of
University of Agriculture, Faisalabad. Peels of both fruits were separated manually, dried in shade at room temperature, ground to fine powder using a laboratory grinder and stored in polythene bags at 4 C until further use. Powdered peels
(20 g) were extracted for 24 h with 200 mL absolute methanol and ethanol, separately at
50 rpm at 30 C. The peel residue was filtered, extracted twice with respective solvent and extracts were pooled.
Solvent was nearly evaporated at low pressure and at 45 C, using a rotary evaporator
(N-N Series, Eyela, Rikakikai Co., Ltd., Tokyo, Japan), and yield of dried extracts was calculated.
Extracts were stored at 4 C for
further analysis.