Several changes in the proportions of the mobile phase components were assayed(acetonitrile-water[80:20,82:18, and85:15,v/v]),and the flow rate was varied between 0.8-1.2mL/min
Several changes in the proportions of the mobile phase components were assayed(acetonitrile-water[80:20,82:18, and85:15,v/v]),and the flow rate was varied between 0.8-1.2mL/min