The purpose of this study was to assign the utilization of Asper Backer bamboo charcoal as activated carbon and pyroligneous acid as an alternative coagulating and antifungal agents for natural rubber sheet production. The effect of chemical activation process of bamboo charcoal was investigated. The physical properties like iodine number, moisture, ash, volatile and fixed carbon contents were measured. The results were found that the bamboo activated carbon has the highest iodine number value of 1398 mg/g which indicating a large of adsorption capacity at the optimum conditions as microwave power of 360 watts, activation time of 15 mins and the ratio of charcoal: phosphoric acid as 1: 4. In addition, the effect of coagulating and anti-fungal activities of pyroligneous acid that is a by-product of Dendrocalamus Asper Backer bamboo charcoal burning was also observed. The comparison properties of pyroligneous acid with the commercial wood vinegar, formic and acetic acids have been measured. The coagulating efficiency of pyroligneous acid exhibited the shortest possible coagulation time at 18.30 mins between acids and latex. Herein, the antifungal efficiency was determined from a fungi growth area only 5% on air dried sheet rubber surfaces. The results were found that the coagulating and anti-fungal properties showed in the following order of commercial wood vinegar > bamboo pyroligneous acid > formic acid > acetic acid according to their acidic and phenolic compound contents.