3.2. Effect of substrate and enzyme loading
Different amount of NH4OH pre-treated fern (pteris) substrate ( 1.25gm/L, 2.5gm/L, 3.75gm/L) having 45-63μm
particle size and different amount of enzyme (10ml/L; 20ml/L; 30ml/L; 40ml/L) was inserted to investigate the
effects The both study was performed maintaining pH 7; temperature 298K. The study shows that 2.5gm/L is
optimum though for 3.75gm/L substrate the glucose concentration is slightly higher than for 2.5gm/L but the
reaction rate is comparatively slow due to substrate to enzyme concentration ratio increased investigated by
Kristensen [15] shown in fig -2.
Again fig-3 shows that the concentration of the reducing sugar increased up to 71 hours for all enzymes loading.
It is observed that 30ml/L enzyme loading is optimum because the reaction rate is too slow for the lower enzyme
concentration again the 40ml/L enzyme comparatively less-efficient than 30ml/L enzyme loading.]