It was used 5 g of pulp in centrifuge tubes and extracted firstly with 20 ml of methanol/water
(50:50, v/v) leaving it in rest at room temperature for 60 min. The tubes were centrifuged at 15,000 rpm for 15 min and
the supernatant was recovered. Following that 20 ml of acetone/water (70:30, v/v) was added to the residue at room temperature for 60 min and centrifuged.