2. Materials and methods
2.1. Chemicals
g-aminobutyric acid standard solution, a-amylase enzyme (ref.
A-3176), amyloglucosidase enzyme (ref. A-7095), pepsin enzyme
(ref. P-7000) and glucose oxidase-peroxidase kit (ref.GAGO-20)
were obtained from Sigma (SigmaeAldrich Co., St. Loius, USA).