The identity of IBV-mCherry was first confirmed by RT-PCR
(Fig. 2A). Total RNA was extracted from mock, IBV WT and IBVmCherry
infected (MOI 1.0) cells at 18 hpi. Expected band sizes of
approximately 1100 and 2075 nts were observed, corresponding
to the WT and mCherry NS vRNA segments, respectively (Fig. 2A).