2.5.4. Respiratory burst activity
The respiratory burst activity of phagocyteswas measured using
the nitroblue tetrazolium (NBT, SigmaeAldrich) assay, according to
the method of Secombes [21] with previously described modifications
[10]. Colour development was measured at 630 nm with
a spectrophotometer. KOH/DMSO was used as blank