Fig. 1. H&E (Mayer–Bennet haematoxylin and eosin-phloxine) histology and in situ hybridization of Enterocytozoon hepatopenaei (EHP) in the infected shrimp. (A) H&E
staining of hepatopancreas tissue of the Penaeus vannamei. Arrows indicate mature spores, stars indicate the plasmodia stage; (B) In situ hybridization of the consecutive
section with a digoxigen-labeled EHP probe. The presence of dark blue precipitates indicates the presence of EHP. (C) H&E staining of P. stylirostris; (D) In situ hybridization
with EHP probe. (E) H&E staining of tail muscle of a P. monodon showing the cotton shrimp disease caused by a Pleistophora-like microsporidium; (F) In situ hybridization with
EHP probe, (insert photo in F): in situ hybridization with a Pleistophora-like microsporidian probe. Scale bars = 25 lm. (For interpretation of the references to color in this
figure legend, the reader is referred to the web version of this article.)