To examine the presence of oil globules in the cytoplasm of the oocytes,selected tissues were fixed in 10% neutral buffered formalin, immersed in 30% sucrose solution overnight
at 4∞C, embedded in optimal cutting temperature compound (Sakura Finetek, Tokyo, Japan),
made into 6mm sections using cryostat at20∞C and stained with Sudan Black B
To examine the presence of oil globules in the cytoplasm of the oocytes,selected tissues were fixed in 10% neutral buffered formalin, immersed in 30% sucrose solution overnightat 4∞C, embedded in optimal cutting temperature compound (Sakura Finetek, Tokyo, Japan),made into 6mm sections using cryostat at20∞C and stained with Sudan Black B
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