For T1 and T2, the peak value of reducing sugar concentration attained to 45.3 g L−1 and 50.8 g L−1, respectively, on hours 12, which was higher than those (45 g L−1 and 42 g L−1) obtained by SSF and SSCF (simultaneous saccharification and cofermentation), respectively, in batch mode reported by Kang et al.