3.4. Fatty acid analysis
The characterization and quantification of fatty acids are shown
in Table 2. The results on lipid composition correspond only to the
free fatty acid fraction. In addition, there is substantial difference
between the current study using GC/FID and Garrote et al. (2007) in
which MS was coupled with GC. Refluxing for 120 min resulted in
the significantly highest amount of total fatty acids, followed by 60
and 30 min (p < 0.05). Although valeric acid was not detected
under this condition, the RD6 rice husk cultivar contained higher
fatty acid contents than a previous report of the rice cultivar grown
in Spain (Garrote et al., 2007). The amount of fatty acid content was
in accord with the extract yield, antioxidant activity and active
principle contents, which were determined using the TPC and TFC
measurements. Correlation between activity and active compounds
were further monitored. The TPCwas most related with antioxidant
activity (r ¼ 0.997), followed by total fatty acid content (r ¼ 0.864),
whereas TFC was not related (r ¼ 0.004). Furthermore, linoleic acid
was positively related with TPC (r ¼ 0.986) and exhibited an antioxidant
response (r ¼ 0.996). Furthermore, linoleic acid from
120 min extraction was significantly higher than 30 and 60 min
(p < 0.05). This report studied Thai rice in particular, and refluxes of
60 and 120 min of the husks resulted in higher contents of heptanoic,
nonanoic, oleic and linoleic acids compared to Spanish rice
(Garrote et al., 2007).
3.4. Fatty acid analysisThe characterization and quantification of fatty acids are shownin Table 2. The results on lipid composition correspond only to thefree fatty acid fraction. In addition, there is substantial differencebetween the current study using GC/FID and Garrote et al. (2007) inwhich MS was coupled with GC. Refluxing for 120 min resulted inthe significantly highest amount of total fatty acids, followed by 60and 30 min (p < 0.05). Although valeric acid was not detectedunder this condition, the RD6 rice husk cultivar contained higherfatty acid contents than a previous report of the rice cultivar grownin Spain (Garrote et al., 2007). The amount of fatty acid content wasin accord with the extract yield, antioxidant activity and activeprinciple contents, which were determined using the TPC and TFCmeasurements. Correlation between activity and active compoundswere further monitored. The TPCwas most related with antioxidantactivity (r ¼ 0.997), followed by total fatty acid content (r ¼ 0.864),whereas TFC was not related (r ¼ 0.004). Furthermore, linoleic acidwas positively related with TPC (r ¼ 0.986) and exhibited an antioxidantresponse (r ¼ 0.996). Furthermore, linoleic acid from120 min extraction was significantly higher than 30 and 60 min(p < 0.05). This report studied Thai rice in particular, and refluxes of60 and 120 min of the husks resulted in higher contents of heptanoic,nonanoic, oleic and linoleic acids compared to Spanish rice(Garrote et al., 2007).
การแปล กรุณารอสักครู่..