A hairy roots culture of Pueraria candollei was established using Agrobacterium rhizogenes
ATCC15834 and grown in half-strength Murashige and Skoog (MS) medium. The
highest production of total isofl avonoids was found to be (36.48 ± 4.09) mg/g dry wt
[(3.39 ± 0.20) mg/g dry wt puerarin, (29.91 ± 3.74) mg/g dry wt daidzin, (1.65 ± 0.09) mg/g
dry wt genistin, (0.76 ± 0.03) mg/g dry wt daidzein, and (0.76 ± 0.03) mg/g dry wt genistein,
respectively]. The total isofl avonoid content in hairy roots of P. candollei was 5.18-fold higher
than that of the native tuber. Effects of sucrose content and medium type on growth
and isofl avonoid production were investigated. 5% (w/v) Sucrose was an optimum content
for the growth and isofl avonoid accumulation in P. candollei hairy roots. Half-strength MS
medium had the highest effect for biomass production whereas woody plant medium had
mostly stimulated isofl avonoid content in hairy roots.