Three 5 × 5 mm pieces of
tissue were taken from the margin of infected
tissues, surface sterilized by dipping in 1%
sodium hypochlorite for 1 minute, immersed in
70% ethanol for 1 minute and rinsed three
times with sterilized water and finally dried in
sterilized tissue paper. Samples were placed on
water agar and incubated at room temperature
(28-30°C)