polyphenolics punicalagin A, punicalagin B, ellagic acid and gallic
acid in pomegranate juice and pomegranate containing products
was developed. This method displays good linearity, good daily
reproducibility, a high recovery rate, and has low limits of detection
(LOD) and quantification (LOQ). Compared to existing methods,
this method offers significant improvements in sample
throughput and facilitates the quantitative determination of four
major polyphenolics in a single run. This method was applied to
investigating the influence of extraction time on the water-based
extraction of pomegranate marc. We found that shorter extraction
times (60 min) resulted in extracts with higher
punicalagin levels. In the future, it may also be possible to use this method to verify the presence of pomegranate metabolites in products,
or to detect adulteration.