Histological Procedures
Five fry from several broods were collected every 2±3
days from 9±65 days PF and thereafter till 90 days PF
collected every 5±7 days. Weight and total body length
were measured. Horizontal serial sections (4 mm) were
®xed in Bouin Holland ¯uid of whole fry at early stages
(from 9±45 days PF) and subsequently (from 45±90
days PF) on either trunk or gonads. We performed a
thorough examination of gonads before differentiation
which were entirely cut into serial sections. Sections
were stained with Regaud's hematoxylin, Orange G
and aniline blue. In addition, sections of 1 mmbelonging
266 H. D'COTTA ET AL.