Point-resolved spectroscopy
sequence (PRESS) was used for spatial localization and signal
acquisition using a protocol set up by Schrauwen-Hinderling et al
(repetition time/echo time, 4000/33 ms; number of signals
averaged fat/water, 64/16) [19]. Water signal was suppressed
using 150 Hz frequency-selective prepulses. The unsuppressed
water signal was measured in the same volume of interest under
the same shimming conditions, and was used as a reference signal
[19]. Spectra were fitted using the Java-based magnetic resonance
user interface (jMRUI) package fitted in the domain using a nonlinear
least-squares algorithm (AMARES) [19,20,21].