Oil samples were collected from Crude Oil Indian Corporation Pvt., Ltd., KK Nagar West, Chennai. Soil sample
were used to analyse the physico-chemical parameters and to isolate the bacteria .Samples were collected at a depth
of 5cm from the surface of the soil. They were collected in sterile polythene bags and tightly packed. They were
then carefully transferred to the laboratory for the analysis and stored at 4°C aseptically before processing.