Abstract
The genome of Thai-isolate of tomato yellow leaf curl virus (TYLCV-THL), a bipartite geminivirus, was cloned and the nucleotide sequence was analysed. The genome designated component A and B are 2751 and 2737 nucleotides long, respectively. The component A contains seven open reading frames (ORFs) with coding potential for proteins of molecular weight more than 10,000 Daltons. The component B has totally three ORFs on both strands. Computer-based analysis to compare nucleotide sequence of TYLCV-THL DNAs and the predicted amino acids with other selected geminiviruses indicated high degree of similarity with geminivirus which infected tomato in Australia and Israel for the component A. The component B showed greater homology of amino acid sequence with those of cassava latent and squash leaf curl geminiviruses.