The poor root system found in mangosteen seedlings represents a significant problem in mangosteen propagation, becauce the roots growth was very slow. In vitro propagation through tissue culture techniques could be an alternative solution to solve the problem. The technique may also help production of more uniform and high quality seedlings. However, until present, rapid shoot proliferation and satisfactory rooting result have not been achieved ( Harahap et al. 2012).
Some groups of plant growth regulator (PGR), such as auxins and cytokinins, are very effective for inducing organogenesis. Zuraida et al. (2011) claimed that 5 mg/L benzyl amino purine (BAP) is required to increase shoot development in pineapple plants during the in vitro stage. Rostika et al. (2008) reported that the highest number of mangosteen axillary buds were formed using a medium containing 3 mg/L benzyl adenine (BA). The objective of this research was to determine the best PGR combination for in vitro shoot and root inductions from several explant types of mangosteen seed.