Assessment of antioxidant enzymes and their scavenging
activity
Antioxidant enzymes activity
One week old plants from various treatments were collected for
assaying antioxidant enzyme activity. One gram of leaf was
homogenized in 10 ml ice-cold 50 mM potassium phosphate
buffer (pH 7.8) in pre-chilled mortar and pestle. Further
homogenate was centrifuged at 5,000 rpm for 10 min at 4°C in
a refrigerated centrifuge. The supernatant was used as an
enzyme source within 12 h of extraction for assaying
Superoxide dismutase (SOD), Catalase (CAT) and Ascorbic
acid oxidase (AOX).