Each culture was previously grown in tubes containing agar for 48 h
at 30 ± 1°C. Each culture was re-suspended in sterile water and
inoculated (volume corresponding to 10% of the volume of the
culture medium, or 4g of dry yeast per liter of pulp) in 250 ml
Erlenmeyer flasks containing 100 ml of sterile banana pulp (121°C
for 15 min, pH 5.0). Flasks were incubated for 24 h at 30 ± 1°C and
150 rev/min. Fermentation tests were conducted in triplicate. The
schematic diagram of the experimental fermentation assays is
shown in Figure 1.