On the basis of previous results concerning the effects of cobalt
hexamine [Co(NH3)6]3+, a known efficient inhibitor of HHR selfcleavage [13], the influence of this compound, and that of its analog ruthenium hexamine [Ru(NH3)6]3+ (generally used as an enzymereducing agent), on the activity of CChMVd-HHR was tested and the results are compared in Fig. 5A. These two molecules are rather good inhibitors of the ribozyme, with IC50 of 84 ± 6 μM and 25 ± 3 μM