Root establishment: When the regenerated auxiliary buds were separated and transferred
to the rooting medium with different hormones, rhizogenesis occurred followed by
enlargement of the stem during 40 days culture. The effects of environmental and medium
factors on rooting were investigated by evaluating the rooting percentage, root length, and
number of roots per plantlet. The combination and concentration of auxin forms
significantly influences the number of roots formed (Table 1). The best rooting and
elongation occurred with treatment 2 that contained 0.1 mg·L
-1
IBA and 0.05 mg·L
NAA
(Fig. 1C). The combination of these two auxins for increasing rooting has previously been