The experimental results lead to the conclusion that black gram
had a high amount of catalase. The Seph 4B-IDA-Zn(II) (IMAC) column
employed for the purification of catalase from sprouted black
gram seeds yielded 39% of catalase with a purification fold of 106.
Boththe SDS–PAGE and Zymogramanalysis resulted thatthe recovered
protein was catalase. Further the kinetic studies based on
determining Km and Vmax values as well as the inhibition studies
were in corroboration with the catalase purified fromother sources.
The physical studies demonstrated the optimum temperature and
pH as 40 ◦C and 7.0, respectively. There by it can be concluded that
the purification method used proves to be a potential approach for
purifying catalase from black gram seeds in a single step.