The evaluation to AOSA of the pigment
First diluted the pigment extract solution and Vc solution
to 10, 50, 100, 150, and 200 times, then tested
the absorption value and calculated the depressing rate
(Table 3). According to the data in Table 3, the optimal
concentrations of the depressing rate were between
40-50% with 100 times dilution of pigment and
150 times dilution of Vc, and the results of AOSA are
shown in Table 4. We could learn from Table 4 that
the AOSA of the pigment was 3 776.31 U mL-1, 1.24
times of Vc (3 042.28 U mL-1) calculated by the
Eq. (5), which proved the pigment extracted from green
wheat bran had a higher activity in active oxygen
scavenging.