Bio-protective these cultures inhibited the growth of Brochothrix thermosphacta, determining an improvement of the microbial and organoleptic qualities of the meat. Bio-protective cultures, which inhibited the spoilage bacteria, were able to reduce the TVB-N to values below 30 mg nitrogen/100 g. The sensory traits of the hamburgers were positively in fluenced by the presence of the bio-protective cultures, as the odours, flavours, and the sticky white slime, that are indicative of deterioration, were not observed in the inoculated samples. The bio-protective cultures evaluated in this study can potentially extend the shelf life up to 12 days and improve the sensory properties of hamburger meat.