The production of ornithine and succinic acid by Corynebacterium glutamicum CGMCC1006-ldhA media
was performed using cane molasses as a low-cost material under aerobic and anaerobic conditions,
respectively. The use of a series fermentation process would reduce both production costs and excess
bacteria. In this work, the ornithine concentration reached 14.93 g L−1 within 72 h when using the optimal
medium containing molasses pretreated with sulphuric acid and coupled with polyacrylamide, whereas a
succinic acid concentration of 13.11 g L−1 was attained after 12 h using amedium containingmolasses pretreated
with sulphuric acid. In fed-batch fermentation, the succinic acid concentration reached 35.13 g L−1
within 54 h when using molasses. The present study suggests that the use of molasses as a raw material
in series fermentation for the production of ornithine and succinic acid by C. glutamicum can significantly
reduce the production costs and the amount of excess biomass.