Five milliliters of T.
longibrachiatum suspension of each concentration and
1 ml of
second stage juvenile suspension containing 100 second stage
juveniles
were pipetted into disinfectant Petri dishes. The control
culture plates each contain 5 ml of sterile water and 1 ml of
suspension containing second stage juveniles. This experiment had
six replicates.
After 24, 48, 72 h of treatment, the percentage of immotile
second stage juveniles of M. incognita was calculated, and immotile