show the effect of ICV injection of ghrelin on food
intake. In ad lib-fed chicks, the injection of 2 and 10 pmol ghrelin
significantly decreased food intake of fed chicks at 30 min
(Fig. 1A). The anorectic effect of 10 pmol ghrelin lasted for
60 min after injection. In 12-h fasted chicks, ghrelin significantly
suppressed food intake: the anorectic effects were more potent
than those of fed chicks (Fig. 1B), 0.4 pmol ghrelin was effective
at 30 min after injection, and the effects of 0.4 and 2 pmol ghrelin
184 T. Tachibana et al. / General and Comparative Endocrinology 171 (2011) 183–188
lasted until 60 min after injection. Injections of 10 pmol ghrelin decreased
food intake throughout the experimental period