For the in vivo study, thirty
Firstly, to determine whether inulin affects HK leucocyte
viability, they were incubated with inulin and 30 ml of
propidium iodide (PI) (400 mg ml1, Sigma) were added to
each sample. The samples were then analysed in a FACScan
(Becton Dickinson) flow cytometer with an argon-ion laser
adjusted to 488 nm.