MaterialsBarley straw was used as the lignocellulosic biomass and was obtained fromthe Jeollabuk-do Agricultural Research and Extension Service, Iksan, Korea. It wasmilled and fractionated to a particle size of <3 mm using sieves and was stored ina plastic container at room temperature. Moisture content (∼5%) was maintainedin the biomass through oven drying. Cellic® CTec II and Cellic® HTec II (Novo Inc.,Bagsvaerd, Denmark) were applied for enzymatic hydrolysis and SSF. All reagents(except sodium hydroxide) were of analytical grade. Sodium hydroxide (DuksanChemical Co. Ltd., Seoul, Korea) was of industrial grade
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