Brewer's grains are the most important by-product of the brewery industry and it is mostly used as a
protein and energy source in animal nutrition. Listeria monocytogenes is a food-borne pathogen can cause
invasive diseases such as meningoencephalitis, sepsis, abortion, and gastroenteritis in humans and
several animal species. The aim of this work was to study the antilisterial activity of lactic acid bacteria
(LAB) isolated from brewer's grains. The incidence of Listeria spp. in brewer's grains was 3.12%. Twentyone
LAB inhibited the growth of the eight strains of L. monocytogenes. The mean inhibition halo of cell
free supernatants of LAB ranged between 11.5 and 24.5 mm. The isolation of lactic acid bacteria with
antilisterial activity from brewer's grains is promising based on their capacity to produce antimicrobial
compounds. The production of antimicrobial metabolites by LAB in the substrate would generate an
unfavorable environment for the growth of the pathogenic bacterium under study