was determined using Ellman's (1959) method. Briefly, 1 mL of 2 mg/mL MP was mixed with 8 mL buffer (0.086 M Tris, 0.09 M glycine, 4 mM EDTA, pH 8), and centrifuged at l0,000 g for 15 min.
was determined using Ellman's (1959) method. Briefly, 1 mL of 2 mg/mL MP was mixed with 8 mL buffer (0.086 M Tris, 0.09 M glycine, 4 mM EDTA, pH 8), and centrifuged atl0,000 g for 15 min.