From Table 1 it is possible to analyze the concentration, yield and purity of genomic DNA extracted
from several species (N = 100). One of the important features of this protocol is the possibility of its
application to a great variety of mollusc species. The average concentration of the total extracted
genomic DNA of all samples was 271.8 ± 64.5 ng·μL−1 (mean ± SE), ranging from
200.7–370.3 ng·μL−1 (min-max). Typical DNA yield ranges from 1000–5000 ng·mg−1 in animal tissue [16].
For the mollusc analyzed in this study, the extraction method generated adequate DNA yield, ranging
from 823.6–5053.8 ng·mg−1 (mean ± SE). Indeed, it is not easy to compare our results with the ones
achieved by other methods and that are already published for mollusc species, as most of them use
different types of tissues such as gills, rectum and mantle. In fact, these tissues are easier for DNA
isolation but in the end the DNA could be contaminated with alien DNA as for example from parasites.
Nevertheless, in recent works published [15,16] with protocols for bivalves DNA extraction, the total
DNA measured ranges between 0.5–250 μg, indicating similar values of high molecular weight DNA
using this protocol, a mean of 27.2 ± 6.5 μg (mean ± SE)