2. Materials and methods
2.1. Sample preparation
In order to examine the optimal reaction conditions on GABA
production, barley bran prepared from the grain of cultivar CDC Fibar harvested in Canada in 2005 was used. The grain of wheat of No. 1 Canada Western Red Spring (1CW) and rice of cultivar
Akitakomachi harvested in Japan in 2006 were also used for preparation of bran. In order to prepare bran, 180 g of whole grain was
applied to the test mill, TM-05C (SATAKE, Japan) and milled up to
108 g of grain weight.