which was stirred using an Ultra Turrax homogeniser for 3 min. Then 15 mL of 1 103 Mol L1 solution of calcium chloride was added, followed by vigorous stirring for 30 s. The mixture was centrifuged (2000 g) for around 15 min. The phases were then separated and evaporated to chloroform phase in a rotary evaporator under reduced pressure at 40 C.