lysozyme (10 mg/mL) and lyticase from Arthrobacter luteus
(1 mg/mL) (all enzymes from SigmaeAldrich, Milan, Italy) and
incubated at 37 C for 2 h. After 30 min incubation at 37 C with
50 mg/mL ribonuclease A (Sigma-Aldrich) and 5 g/L sodium
dodecyl sulphate (SDS), and a second incubation at 55 C for 30 min
with 10 mg/mL proteinase K (SigmaeAldrich),