Conclusion
The results described in this research provided the possibility
of discriminating Chinese herbal medicines according
to their geographic regions using 2D-NIR correlation spectroscopy
instead of 2D IR spectra. Practically, direct NIR
measuring of herbal medicines made the measurement much
more convenient, and can be applied to the quality control of
the Chinese medicine manufacture. In this research, we took
barely any pretreatment steps of samples owing to the advantages
of NIR spectra, only drying samples at 60 ◦C for 12 h.
Since this temperature was widely used in the discrimination of
Traditional Chinese medicine by 2D mid-IR correlation spectroscopy.
Besides, the drying temperature of 40 ◦C for 24 h
and 50 ◦C for 18 h, and the method of vacuum freeze-drying
(100 Pa, −10 ◦C, 6 h) was also studied. All of these drying
temperatures yield the same discriminating results. Finally, we
choosed 60 ◦C for 12 h, since the thermo-state oven is available
in every lab. The same experiment was carried out in
6 days by the same person, and was carried out by four different
coworkers in our team, with the same material, methods
and instruments described in Section 2. The same results can
be got. It showed high reliability and reproducibility of this
method. A similar research strategy can be directly applied
to the study of other important herbal medicines for the same
purpose.
Conclusion
The results described in this research provided the possibility
of discriminating Chinese herbal medicines according
to their geographic regions using 2D-NIR correlation spectroscopy
instead of 2D IR spectra. Practically, direct NIR
measuring of herbal medicines made the measurement much
more convenient, and can be applied to the quality control of
the Chinese medicine manufacture. In this research, we took
barely any pretreatment steps of samples owing to the advantages
of NIR spectra, only drying samples at 60 ◦C for 12 h.
Since this temperature was widely used in the discrimination of
Traditional Chinese medicine by 2D mid-IR correlation spectroscopy.
Besides, the drying temperature of 40 ◦C for 24 h
and 50 ◦C for 18 h, and the method of vacuum freeze-drying
(100 Pa, −10 ◦C, 6 h) was also studied. All of these drying
temperatures yield the same discriminating results. Finally, we
choosed 60 ◦C for 12 h, since the thermo-state oven is available
in every lab. The same experiment was carried out in
6 days by the same person, and was carried out by four different
coworkers in our team, with the same material, methods
and instruments described in Section 2. The same results can
be got. It showed high reliability and reproducibility of this
method. A similar research strategy can be directly applied
to the study of other important herbal medicines for the same
purpose.
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